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Lab Safety Checklist

Work through this before an OESO walkthrough.

Lab Safety Checklist

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Lab Safety Checklist - to be completed by the lab before OESO walkthrough		Date
PI Name	Emma Chory	Safety Contact Name	Ryan Boileau
Department	Biomedical Engineering	Safety Contact Phone	5036790739
Box #		Safety Contact Email	ryan.boileau@duke.edu
Your buildings & rooms	CIEMAS 2393, CIEMAS 2208,CIEMAS 2216, CIEMAS 2398
Shared buildings & rooms (incl. cold rooms)	CIEMAS 2208, CIEMAS 2398

Documentation and Training

1	Is the list of lab members in the LSWMS up to date?				Yes	No
2	Which of the following can personnel easily access (via cloud drive, hardcopy, web, etc.)?
	Duke University Safety Manual Duke Laboratory Safety Manual Radiation Safety Manual Duke Chemical Hygiene Plan Reproductive Health Policy Safety Data Sheets (SDSs)	Report of Work-Related Injury or Illness Form OESO Emergency Response Guide & Webpage, including Exposure Hotline - Bio, Chem, or Rad – 919-684-8115 Blood Borne Pathogens Exposure Control Plan Minors/Non-Employees in the Workplace Policy Training records/Written procedures for machine tools	Lab Chemical Waste Management Practice P-List of acutely hazardous chemical wastes Drain Disposal Guidelines - (flyer) Aerosol Can Disposal Policy Eyewash/drench hose flushing records Appropriate Information Sheets
3	Please indicate how many copies of the OESO Emergency Response Guide flipchart you need:
4	Is the presence of food and drink prohibited in appliances used for potentially infectious materials or hazardous chemicals through training and labeling of refrigerators, freezers, and microwaves?			N/A^[1]	Yes	No
5	Are eating, drinking, applying cosmetics, and handling contact lenses prohibited in areas where there is any risk of exposure to potentially infectious materials or hazardous chemicals?			N/A	Yes	No
6	Are employees trained on safe use and proper clothing/PPE for autoclaves? (See also info sheet, hazard alert, and training checklist.)			N/A	Yes	No
7	Do you have any minors or non-employees in your lab?				Yes	No

Fire Safety

1	Are all lab members familiar with the location of fire alarm pull stations and fire extinguishers?		Yes	No
2	Are open flames/Bunsen burners only used outside biosafety cabinets (BSCs) and/or fume hoods?	N/A	Yes	No
3	Are combustible materials such as paper removed from the lab bench when using open flames/Bunsen burners?	N/A	Yes	No
4	Does the lab avoid storing flammable chemicals in "Not Approved” fridges/freezers?	N/A	Yes	No
5	Are extension cords used on equipment for only less than 30 days in a row? This is NOT regarding power strips.	N/A	Yes	No
6	Do all workers know where the outside Emergency Assembly Point is located? (See Site-Specific Fire Plan Part I)	N/A	Yes	No

General Safety and Personal Protective Equipment (PPE)

1	Are there any procedures that require PPE in your lab?						N/A	Yes	No
2	Have all lab members been provided with instructions about the use, care, and storage of PPE?						N/A	Yes	No
3	Which types of PPE are available in your lab:
	Nitrile gloves Alternative gloves (for anyone allergic) Neoprene gloves Cryogen gloves (cold/liquid resistant) Autoclave gloves (heat resistant) UV/IR eye protection	Goggles Safety glasses Surgical Masks Face shields				Standard lab coat Chemical-resistant apron Disposable apron Other: None
4	If lab coats are used and become contaminated/dirty, how are they cleaned:
5	Please list the first and last name of lab members who use respirators, if any:
6	Are your freezers and fridges continuously monitored for their temperature?						N/A	Yes	No
7	Are you using an autoclave for sterilization? Yes No If so, do lab members AVOID adding water to the bin?						N/A	Yes	No
8	Are all uncontaminated sharps (e.g., broken glassware and Pasteur pipets) disposed of in an appropriately lined puncture-resistant container?						N/A	Yes	No
9	What type of contaminated sharps are generated as waste?		biological	chemical	None
10	Are fridges/freezers, cold rooms, and other storage areas periodically checked for their contents?						N/A	Yes	No

Chemical Safety

1	When procuring chemicals, does the lab select the least hazardous chemical or container possible for a procedure?	N/A	Yes	No
2	For situations that call for the transport of chemicals, are secondary leak-proof containers used?	N/A	Yes	No
3	Please list all peroxide-forming chemicals (e.g., isopropanol, diethyl ether, tetrahydrofuran), if any:
4	Please list all P-Listed chemicals in your lab, if any:
5	Are hazardous and irritating powders handled/weighed according to safe work practices?	N/A	Yes	No
6	Does your lab review chemicals in storage to sort out obsolete chemicals?	N/A	Yes	No
7	Is “Universal Waste” (e.g., used batteries or mercury thermometers) generated or stored in your lab?	N/A	Yes	No
8	Are your lab members shipping chemicals off-campus?	N/A	Yes	No
9	Please describe how your lab members use liquid nitrogen and how much they dispense at a time, if applicable:
10	Please describe how often and how long you run 3D printers in your lab:

Biological Safety

1	Please list the biological materials such as toxins, tissues, cell lines, bacteria, viruses, and fungi used in your lab:
2	Where are whole plants grown, if any:
3	Please list everyone who is actively working with human-derived materials in your lab:
4	Please list everyone working with other BSL-2 materials:
5	Select any of the following materials your lab members are using, if any: rDNA Adeno-associated viruses (AAV) Adenovirus Baculovirus Lentivirus Retrovirus Other viral vectors:
6	Select current disposal methods for biological waste, if such waste is generated:
	Autoclaving; location: Incineration offsite; location:	Chemical disinfection Other:
7	Are work surfaces wiped down immediately with an appropriate disinfectant at the end of each procedure or immediately following a spill?		N/A	Yes	No
8	Are all biological materials transported in closed, durable, leak-proof containers within and outside the lab?		N/A	Yes	No
9	Are needle-locking syringes/single use needle-syringe units used with infectious agents?		N/A	Yes	No
10	Do all vacuum lines used with biological materials have in-line filters and disinfectant traps?		N/A	Yes	No
11	Please list everyone responsible for completing shipping papers or packaging biological material and/or dry ice:
12	Is Employee and Occupational Health and Wellness (EOHW) contacted for immunizations, surveillance, and treatment of occupational exposures?		N/A	Yes	No
13	Is your work with human subjects or clinical research participants approved by the Institutional Review Board (IRB)?		N/A	Yes	No
14	Are clinical specimens (saliva, blood, etc.) collected from research subjects?		N/A	Yes	No

Animal Use

1	Please list all animal species used by lab members:
2	Please select the method of controlling anesthetic gas exposure:
	Chemical fume hood Vacuum scavenging	Captured with charcoal canister e.g. F/Air (used charcoal canisters need to be submitted as chemical waste)	Released into lab Other:
3	Does the lab create or work with agents used in animals at either Duke or another location?			Yes	No

Any other questions, concerns, and comments

[1] N/A means Not Applicable

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