Chemical Hygiene Plan

Laboratory Unit

Main Lab: CIEMAS, 2391, 2397

TC Room: CIEMAS 2216

Robot Room: CIEMAS 2208

Cold Room: CIEMAS 2304

Principal Investigator:

Name: Emma Chory

Office Location: CIEMAS. Room 2353b

Work (cell) Phone Number: (203) 273-3711

Alternate Phone Number: (984) 327-8702

Department Chair:

Name: Sharon Gerecht , PhD

Office Location: 1427 FitzPatrick CIEMAS

Work Phone Number: (919) 660-5128

Laboratory Safety Coordinator (LSC):

Name: Ryan Boileau, PhD.

Office Location: CIEMAS 2393

Work Phone Number: (503) 679-0739

Alternate Phone Number:

Laboratory Chemical Hygiene Officer

Name: Ryan Boileau

Title: Postdoctoral Fellow

Office Location: CIEMAS 2393

Work Phone Number: (503) 679-0739

Alternate Phone Number:

Check below all items that are appended to this plan. Unless otherwise indicated, copies of appended documents should be sent to OESO when this plan is requested.

• List of particularly hazardous and high risk chemicals (Required unless listed on page 2, or unless page 2 indicates that there are no particularly hazardous or high risk chemicals in the lab)
• Customized SOPs noted on pages 4 and 5, if any
• Summary of Chemical High Risk Procedures and Documentation of Approval, and hazard assessments or lab-specific SOPs for each high-risk procedure (Required unless page 6 indicates no High Risk Procedures)
• Laboratory Emergency Response materials (Required unless listed on page 9)
• Laboratory-specific chemical hygiene training documentation (to be kept in lab only – copies do not need to be sent to OESO)

Every lab employee and student should be instructed on how to access and understand Safety Data Sheets. SDSs for our chemicals can be found:

• In this laboratory, located or,
• In the departmental file, located or,
• On a personal or networked computer, located Chory Lab Safety Documents (link) and also available through the chory lab website portal. It is additionally linked in the lab handbook, but is password protected in the handbook, because we have made the handbook publicly available.
• Using the internet Safety Data Sheet database service (CCOHS), which can be accessed through http://www.safety.duke.edu/occupational-hygiene-safety/sds-resources.
• Other:

Backup plan for electronic SDSs: In the case of power or internet outage, contact the manufacturer to have an SDS faxed, or call OESO at 919-684-2794 or 919-684-5996 for assistance.

List below the particularly hazardous and high risk chemicals (Duke Hazardous chemicals link) used in this lab, or attach a list.

Chory Lab hazardous/high-risk Chemicals:

• Paraformaldehyde
• Chloroform
• Phenol
• Trypan Blue 0.4% Solution
• Trizol
• Chloramphenicol
• Kanamycin Sulfate
• Hygromycin B

• The list of particularly hazardous and high risk chemicals is attached.
• We have reviewed our inventory using OESO’s GHS Lookup Tool, SDSs, other relevant sources of information, and the GHS criteria for particularly hazardous and high risk chemicals, and have determined that our lab has no particularly hazardous or high risk chemicals.

Lab-Specific strategies

General strategies for controlling chemical exposures are described in the “Safe Use of Chemicals” section of the University Chemical Hygiene Plan, in the Laboratory Safety Manual.

List below any general lab guidelines that are more stringent than the above-referenced section:

1. Use the chemical fume hood when working with hazardous chemicals and Particulary Hazardous Substances (PHS). Dispose of hazardous chemicals and PHS wastes safely and properly.
2. Wear a lab coat, gloves and safety glasses/ goggles when working with hazardous chemicals and PHS.

Indicate below strategies for safe use of engineering controls in the lab:

• Our lab has a chemical fume hood.

• Look for certification date within the last year on sticker located August 2022
• Verify that hood is under negative pressure by doing the following:

• Check digital monitor for flow rate between 80 and 120 fpm. (When sash is at maximum safe height indicated on hood, flow rate should be close to that shown on most recent certification sticker.)
• Check magnehelic gauge to verify that pressure needle lines up closely with set point.

• Other:

Position sash correctly for work:

• Hood sash moves vertically – keep sash in lowest practical position while working. Sash must come down to shoulder height or lower.
• Hood has combination sash.

• For maximum flexibility, route tubes and cords under airfoil or through access at side of hood. If this is not possible, route these connections under the sash. Avoid running tubes or cords between horizontal sash panels.
• Keep horizontal panels closed and move sash vertically during work. Keep sash in lowest practical position while working. Sash must come down to shoulder height or lower.

OR — Close sash vertically:

• Place one sash panel between body and the work in the hood. Work with arms reaching around this sash panel.
• Our lab uses a biological safety cabinet for handling of powdered chemicals or water-based solutions/suspensions. These hoods are used for Tissue Culture solutions but no powdered chemicals are used here.

• Our lab has “snorkel” exhaust to remove hazardous vapors from the benchtop. The snorkel must be placed as close as possible to the point of contaminant generation (generally within 4 – 6”).
• Our lab has other local exhaust. Safe use instructions are included below.

Standard Operating Procedures (SOPs) and Safety Guidelines

Refer to the Duke Lab Safety Manual, Chemical Safety Section, “Safe Use of Chemicals” chapter for requirements for Standard Operating Procedures.

Mark below the generic and/or lab-customized SOPs that are relevant for this lab. Generic SOPs and guidelines may be referenced online (https://www.safety.duke.edu/laboratory-safety/chemical-hygiene/chemical-sops) or printed. Customized SOPs must be appended to this document.

• 
  This lab does not have or need any generic or customized SOPs.

Chemical High Risk Procedures

Chemical high risk procedures are lab procedures that pose significant risk of serious injury or major property damage if a malfunction were to occur (such as a utility outage, runaway reaction, container failure, or chemical spill/release) and/or which require any of the following:

• Engineering controls more specialized than good room ventilation, chemical fume hoods, biological safety cabinets and/or local exhaust such as snorkel or canopy hoods.^[1]
• Personal protective equipment in addition to gloves, lab coats, eye/face protection and/or chemical or thermal protective aprons or sleeves.
• Chemical-specific first aid treatments or antidotes.

Contact OESO’s Occupational Hygiene and Safety Division at 919-684-5996 if you have questions regarding Chemical High Risk Procedures or if you need OESO permission (as indicated in list below).

Certifications

The following Chemical High Risk Procedures require written approval from the PI and OESO. The date and form of this written approval must be noted below. A written hazard assessment or lab-specific SOP, approved by the PI and kept with this plan, is required for all high risk procedures.

The following Chemical High Risk Procedures require a hazard assessment and written approval from the PI. In some cases, the PI may seek or the Department may require Departmental review. This review/approval must be documented in writing on the hazard assessment/lab-specific Standard Operating Procedure and summarized below.

In addition, any scale-up of a previously approved high-risk procedure will require a new hazard assessment or new lab-specific SOP and written approval by the PI. This section of the lab-specific Chemical Hygiene Plan must be updated when scale-ups have been approved. OESO review/approval will be required for scale-ups of procedures that originally required their approval. The need for Departmental review/approval will be based on Departmental criteria.

Work Practices Requiring Prior Approval

Some laboratory work may not meet the definition of “high risk procedures” above, but may introduce additional risk because of when and/or how the work is conducted.

The following work practices require prior approval of the principle investigator or laboratory safety coordinator/chemical hygiene officer. Detail here if these scenarios are applicable and, if so, how approval will be documented in this lab:

Working alone: None

Unattended Experiments: None

• Our laboratory is a registered chemical waste generator and

• Chemical waste barcode stickers are stored at a place accessible in lab and known by all lab members.
• Waste accumulation stickers are stored in Chemical room 2398 by door and at a separate location in the main lab space. When chemical waste is generated put in an appropriate container, label with the type of waste and open date (use tape). Store in chemical fume hood so it can be disposed by the lab member leading chemical waste.
• When waste needs to be submitted, the following person(s) can submit a waste pickup request through the Laboratory Safety and Waste Management System: Ryan Boileau

• Our laboratory does not generate chemical waste that needs to be picked up by OESO Environmental Programs.
• Our laboratory is off-site and chemical waste is handled as follows:

For general emergency procedures for on-campus labs, see the Laboratory Emergency Response & Incident Reporting Guide (flip chart) and/or the Emergency Response section of the Duke Chemical Hygiene Plan (in the Lab Safety Manual).

List below or attach any specific emergency procedures for this lab. (For example, powering off certain equipment, different chemical spill instructions for off-campus labs, etc.)

• Lab Emergency Procedures are attached.

Our lab’s Emergency Assembly Point location is: Bostock Library/Perkins Library walkway

(For campus buildings, see https://www.safety.duke.edu/fire-life-safety/site-specific-fire-plans to determine the Emergency Assembly Point.)

Spill cleanup supplies are located: Room 2398 top shelf of chemical cabinet and underneath bench with the balances.

Types available (acid, base, solvent, combo, etc.): The kit is approved to clean up acids, bases solvents and unknown liquid (Fisher Scientific: Acid, Caustic and Solvent Spill Kit, Acid, Caustic and Solvent Spill Kit, Cat# S20636 & Uline: Universal Bag Spill Kit - 5 Gallon, Cat #S12200).

Usage information: Kit contains absorbant pads and socks that can absorb up to 5L of liquid. Once pad or socks are used to clean up a spill, place in a new plastic bag, date the bag, label as waste (include the type of waste on the label), and leave the bag near the fume hood for disposal.

By signing and dating here the Laboratory Chemical Hygiene Officer and Principal Investigator certify that this Laboratory-Specific Chemical Hygiene Documentation is accurate and that it effectively provides for the chemical safety of employees and students in this laboratory.

Principal Investigator or Laboratory Director

Emma Chory 8/17/2023

Laboratory Chemical Hygiene Officer (if other than PI)

Ryan Boileau 8/21/2023

By signing and dating here, the Laboratory Chemical Hygiene Officer certifies that the required annual review (and update, if needed) of the Laboratory-Specific Chemical Hygiene Plan has been completed, and that it continues to be accurate and to effectively provide for the chemical safety of employees in this laboratory.

(Copy this form and have each member of your lab sign one before beginning work with chemicals.)

I have received information and training on the subject of chemical hygiene, including:

• I have read the Duke University Chemical Hygiene Plan (Section 3 of the Duke Laboratory Safety Manual).
• I am familiar with the Duke University Safety Manual (https://www.safety.duke.edu/safety-manuals/university-safety-manual) and have reviewed the Hazardous Materials Chapter in that manual.
• I have read the Laboratory-Specific Chemical Hygiene Plan for my laboratory, including the generic SOPs/guidelines and customized SOPs indicated in this plan. I am familiar with the contents of relevant SOPs and guidelines and agree to follow the written procedures.
• I have been given the opportunity to read the OSHA Lab Standard, 29 CFR 1910.1450 (http://tinyurl.com/ov9p27g) and its appendices.
• I have been instructed on how to locate important reference materials, such as those containing hazard information about chemicals and safe handling, storage, and disposal practices for the chemicals found in this laboratory.
• I know where/how to locate Safety Data Sheets for chemicals in this laboratory.
• I have been apprised of the physical and health hazards of chemicals in this laboratory, and am aware of the
• Permissible Exposure Limits (or, if there is no PEL, other recommended exposure limits), and
• Signs and symptoms associated with exposures to chemicals used in this laboratory.
• I am aware of the engineering controls, work practices, emergency procedures, and personal protective equipment needed to protect myself from the hazards in the laboratory.
• I am aware that Duke’s Occupational and Environmental Safety Office may be contacted to evaluate chemical exposure if needed.

Print Name: Date:

Signature:

Laboratory-specific chemical hygiene documentation should be reviewed by each member of the lab at least every year, or whenever there is a revision to the documentation (such as a new or revised SOP).

This signature page certifies review of the following:

• New or revised SOPs or guidelines: All the PHS or High Risk Chemical SOPs listed in the CHP for 2022
• Updates to Chemical Hygiene Plan: New PHS or High Risk Chemicals Added
• Annual review of Chemical Hygiene Plan and relevant SOPs :

[1] More specialized engineering controls include (but are not limited to) inert-atmosphere glove boxes used for employee safety, ventilated gas cabinets, oxygen monitors, and/or toxic gas monitors.